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Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304, Trial Size

产品编号:4096408
规格:clone CMA304, from mouse
包装规格:10 μg
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
409640810 μg1820
产品别名

Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304, Trial Size

H3K4me3, Histone H3 (trimethyl K4), H3 histone family, member T, Histone 3, H3, Histone cluster , , H3

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H3 (Lys4) is is found at the 5′ end of active genes and contributes to transcriptional activation by recruiting chromatin-remodelling enzymes. Accordingly, the inactive X chromosome is largely devoid of this mark.
Specificity【特异性】
This antibody specifically recognizes Histone H3 trimethylated at Lys4. The antibody binding specificity allows for modifications of Lys91.
Broad species cross-reactivity is expected, based on sequence homology.
Immunogen【免疫原】
Epitope: Trimethylated Lys4
Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, trimethylated on Lys4, conjugated to KLH.
Application【应用】
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1

Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1

Multiplexing:
This antibody specifically recognizes histone H3 trimethylated on Lys4 by Luminex® assay.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304, Trial Size is a mouse monoclonal antibody for detection of Trimethyl Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in ELISA, ICC, IP, WB, Mplex, DB.
Quality【质量】
Western Blot Analysis:
0.5 - 2 μg/mL (1:500-2000) dilution of this antibody detected trimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract.
Physical form【外形】
Purified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
Format: Purified
Protein G Purified
Analysis Note【分析说明】
Control
HeLa Acid extract lysate
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
Luminex is a registered trademark of Luminex Corp
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
CMA304, monoclonal
species reactivity
human, vertebrates
technique(s)
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
multiplexing: suitable
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NM_003493
UniProt accession no.【UniProt登记号】
Q16695
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
approx. 17 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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