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Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size

产品编号:4096390
规格:Upstate®, from rabbit
包装规格:20 μg
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
409639020 μg1820
产品别名

Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size

H3K9Ac, Histone H3 (acetyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Specificity【特异性】
This peptide sequence is identical in a wide range of animal and plant species.
Immunogen【免疫原】
KLH-conjugated, synthetic peptide (ARTKQTA RAcKSTG-C) corresponding to amino acids 1-12 of Histone H3.
Application【应用】
Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9 (H3K9ac). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ChIP, ChIP-seq, FC. Small pack size is ideal for screening applications.
Western Blot Analysis:
A431, C2C12 lysate, HEK293 lysate, Hela
lysate, HepG2 lysate , Jurkat lysate , L6
lysate, and NIH/3T3 lysate were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-ANTI-ACETYL HIS H3 (1:500 dilution of a representative lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 06-942-S, Anti-acetyl-Histone H3 (Lys9) at a (1:500) dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.

Dot Blot Analysis:
0.5 μg/mL of a previous lot detected 50 ng of mono-acetylated peptide corresponding to position 9 in the N-terminal sequence of Histone H3 (Catalog # 12-358), but not the monoacetylated peptide corresponding to acetyl-lysine at position 14 (Catalog # 12-359) or unacetylated Histone H3 (Catalog # 12-357).

Immunoprecipitation:
Not determined.

Immunocytochemistry:
Not determined.
Quality【质量】
Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected ANTI-ACETYL HIS H3 on 10 μg of HEK293 lysates.
Physical form【外形】
Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Format: Purified
Protein A purified
Analysis Note【分析说明】
Control
TSA-treated HeLa and NIH/3T3 cells.
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
eukaryotes, mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
dot blot: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NM_003493
UniProt accession no.【UniProt登记号】
Q16695
shipped in【运输】
wet ice
Gene Information
human ... HIST1H3F(8968)
产品说明
Target description【目标描述】
Approx. 17 kDa
【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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