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Anti-glutathione p53 (Cys141) Antibody
产品编号: | 4096370 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-glutathione p53 (Cys141) Antibody
Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, p53 antigen, p53 transformation suppressor, p53 tumor suppressor, transformation-related protein 53, tumor protein p53
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Cellular tumor antigen p53 is a member of the p53 family and is found in the cytoplasm, nucleus, and endoplasmic reticulum. It functions as a tumor suppressor within a variety of tumors by either stimulating apoptosis or growth arrest in deference to cell type and physiological factors. p53 serves as a trans-activator, negatively regulating cell division during cell cycle regulation. It is also thought to be involved in the cross-over for Notch signaling. Defects in p53 expression have been implicated in several diseases including; choroid plexus papilloma, lung cancer, head/neck squamous cell carcinomas, esophageal squamous cell carcinoma, Li-Fraumeni syndrome, and hereditary adrenocortival carcinoma. Mutations of the p53 protein have some characteristic features: a) Most of them are missense point mutations giving rise to an altered protein function, and b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation. |
Specificity【特异性】 | This antibody recognizes p53 when glutathionylated at Cys141. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human p53 glutathionylated at Cys141. Epitope: p53 glutathionylated at Cys141 |
Application【应用】 | Research Sub Category Transcription Factors Anti-glutathione p53 (Cys141) Antibody is a rabbit polyclonal antibody for detection of glutathione p53 (Cys141) also known as Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53 & has been validated in WB, IP, IHC, ICC. Research Category Epigenetics & Nuclear Function Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Dr. Kalkunte S. Srivenugopal, Department of Biomedical Sciences, Texas Tech University Health Sciences Center, 1406 S. Coulter, Amarillo, TX 79106.) Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Yusef, M, et al. (2010). Free Radic Biol Med. 49(5):908-917.) Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Yusef, M, et al. (2010). Free Radic Biol Med. 49(5):908-917.) |
Quality【质量】 | Evaluated by Immunohistochemistry in prostate adenocarcinoma tissue. Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected glutathione p53 in prostate adenocarcinoma tissue. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control Prostate adenocarcinoma tissue |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | monkey (100% sequence homology), mouse (85% sequence homology), marmoset (100% sequence homology), rabbit (100% sequence homology), rat (92% sequence homology), rhesus macaque (100% sequence homology), ground squirrel (100% sequence homology), orangutan (100% sequence homology), chimpanzee (100% sequence homology), hamster (100% sequence homology) |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_000537 |
UniProt accession no.【UniProt登记号】 | P04637 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~53 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |