Anti-acetyl-p53 (Lys120) Antibody

32160702

NA.41

p53 is a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers.

This antibody recognizes the AXIN1 interacting domain of p53 acetylated at Lys120.
Other homologies: Mouse, Rat, Bovine, Feline, Zebrafish, and Equine (88% sequence homology).

Epitope: AXIN1 interacting domain
KLH-conjugated linear peptide corresponding to the AXIN1 interacting domain of human p53 acetylated at Lys120.

Research Sub Category
Transcription Factors
Anti-acetyl-p53 (Lys120) Antibody is a highly specific rabbit polyclonal antibody, that targets p53 & has been tested in Dot Blot & IP.
Research Category
Epigenetics & Nuclear Function
Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in camptothecin treated HCT116 cell lysate.

Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in p53 transfected H1299 human lung cotransfected with or without hMOF (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College).

Immunoprecipitation Analysis: 1.5 µg or 4.5 µg from a representative lot immunoprecipitated endogenous acetyl-p53 in HCT116 cell lysates, which were treated with a vehicle (DMSO) or camptothecin (CPT) (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College).

Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in H1299 cell lysates, which were transfected with either wild type p53, the 9 K/R mutant (all lysine residues except Lys120 mutated to arginine), the 10 K/R mutant (all lysine residues mutate including Lys 120), or empty vector, with or without hMOF (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College).

Evaluated by Dot Blot in non-acetylated and acetylated p53 peptides.

Dot Blot Analysis: 1 µg/mL of this antibody detected acetyl-p53 in acetylated p53 peptides

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
Non-acetylated and acetylated p53 peptides

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

sheep (based on 100% sequence homology), canine (based on 100% sequence homology), rabbit (based on 100% sequence homology), porcine (based on 100% sequence homology)

dot blot: suitable
immunoprecipitation (IP): suitable

NP_000537

P04637

wet ice

~43 kDa calculated. Many isoforms are known to exist. This antibody is predicted to react with isoform 1 (44 kDa), isoform 2 (38 kDa), isoform 3 (39 kDa), isoform 4 (39 kDa), isoform 5 (34 kDa), and isoform 6 (34 kDa).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1