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Anti-Tau Antibody, a.a. 210-241, clone Tau-5
产品别名
Anti-Tau Antibody, a.a. 210-241, clone Tau-5
G protein beta1/gamma2 subunit-interacting factor 1, Neurofibrillary tangle protein, Paired helical filament-tau, microtubule-associated protein tau, microtubule-associated protein tau, isoform 4
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Microtubule Associated Proteins, or MAPS, bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I, II. Type II MAP includes MAP2, MAP4, and tau and are found in nervous tissue. Six tau isoforms exist in brain tissue, and they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (allowing it to bind to the negatively-charged microtubule). The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains. |
Specificity【特异性】 | Tau from bovine, rat and human. |
Immunogen【免疫原】 | Bovine tau Epitope: a.a. 210-241 |
Application【应用】 | Research Category Neuroscience Immunohistochemistry: A previous lot of this antibody was used in immunohistochemistry. Immunoblot: Recognizes phosphorylated and non-phosphorylated isoforms of tau ranging in size from 45-68 kDa. Optimal working dilutions must be determined by end user. Research Sub Category Neurodegenerative Diseases Anti-Tau Antibody, a.a. 210-241, clone Tau-5 detects level of Tau & has been published & validated for use in IH & WB. |
Quality【质量】 | Routinely evaluated by Western Blot on PC12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected Tau on 10 μg of PC12 lysates. |
Linkage【联系】 | Replaces: MAB10417 |
Physical form【外形】 | Unpurified mouse monoclonal IgG1 ascites fluid containing no preservatives. Unpurified |
Analysis Note【分析说明】 | Control Brain Tissue, PC12 cell lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | ascites fluid |
antibody product type | primary antibodies |
clone【克隆】 | Tau-5, monoclonal |
species reactivity | bovine, mouse, human, rat |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1 |
NCBI accession no.【NCBI登记号】 | NM_005910.3 |
UniProt accession no.【UniProt登记号】 | P10636 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | 45-68 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |