您的位置:首页 > 产品中心 > Anti-CNPase Antibody, clone 11-5B
Anti-CNPase Antibody, clone 11-5B
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | The enzyme 2′, 3′-cyclic nucleotide 3′-phosphodi-esterase (CNP) is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system (Sprinkle, 1989). By virtue of this cell-specific expression, CNP is recognized as a characteristic marker for these two myelin-producing glial cell types (Sprinkle, 1989; Kim et al., 1984; Sheedlo & Sprinkle, 1984; McMorris et al., 1984). Beyond its enzymatic activity of cleaving the 2′, 3′-cyclic terminus of nucleotides (Sprinkle, 1989), the physiological role of CNP is still under investigation. CNP activity has been correlated with myelin and myelin formation, and a dramatic decrease in CNP activity is associated with demyelinating diseases such as multiple sclerosis (Sprinkle, 1989). This enzyme is composed of two proteins, CNP1 (46 kD) and CNP2 (48 kD) (Sprinkle, 1989; Sprinkle et al., 1987). Although the ratio of CNP1/CNP2 may vary from species to species, their shared primary sequence is conserved phyIogenetically. CNP has recently been localized to human chromosome 17 by amplification of somatic cell hybrid DNA using the polymerase chain reaction (PCR) and by Southern blotting of Hind Ill genomic DNA digests (Sprinkle et al., 1991). Since anti-CNP reacts with a highly conserved region of the enzyme, it can be considered as pan-anti-CNP (Sprinkle et al., 1987). Anti-CNP can be used as a marker to identify Schwann cells and oligodendrocytes in cell cuIture and in tissue sections, as well as to localize CNP in cell membrane fractions. As CNP is expressed relatively early in postnatal development, anti-CNP is especially useful for the early identification of oligodendrocytes. |
Specificity【特异性】 | Anti-CNP reacts with both CNP1 and CNP2 in many species. |
Immunogen【免疫原】 | Purified human 2’, 3’-cyclic nucleotide 3’-phosphodiesterase |
Application【应用】 | Immunohistochemistry: A previous lot of this antibody was used in IH (10 μg/mL antibody, prepared fresh daily). Immunocytochemistry: A previous lot of this antibody was used in IC (10 μg/mL antibody, prepared fresh daily). Optimal working dilutions must be determined by end user. This Anti-CNPase Antibody, clone 11-5B is validated for use in IC, IH, IH(P), WB for the detection of CNPase. |
Quality【质量】 | Evaluated by Western Blot on Mouse brain lysates. Western Blotting Analysis: 1:500 dilution of this antibody detected CNPASE 1/2 on 10 µg of Mouse brain lysates. |
Physical form【外形】 | Format: Purified Protein A purified Purified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer, pH 7.6, 0.25M NaCl with 0.1% sodium azide. |
Analysis Note【分析说明】 | Control Oligodendrocyte culture, Brain lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 11-5B, monoclonal |
species reactivity | rabbit, canine, rat, human, bovine, sheep, pig, mouse |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1 |
NCBI accession no.【NCBI登记号】 | NM_033133.4 |
UniProt accession no.【UniProt登记号】 | P09543 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | 48 & 46 kDa |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |