Anti-EGR1 Antibody, clone 16A10.1

32160702

NA.41

Early growth response protein 1 (UniProt P18146; also known as AT225, EGR-1, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24) is encoded by the EGR1 (also known as KROX24, ZNF225) gene (Gene ID 1958) in human. EGR-1 is the founding member of the early growth response (EGR) family of Cys2-His2-type zinc finger transcription factors that share a high homology in their DNA-binding region. EGR-1 is ubiquitously expressed in many cell types and binds to a consensus DNA motif (GCGGTGGGCG) in target genes, including c-Myc, cyclins D2 & G2, p19, TNF, IL-2, CD44, and ICAM-1. EGR1 is known as an immediate-early response gene due to the rapid kinetics of its induction by various signals, including growth factors, cytokines, and stress. Human EGR-1 is a 543-amino acid protein with three C2H2-type Zinc fingers (a.a. 338-362, 368-390, 396-418).

GST-tagged recombinant protein corresponding to an internal fragment of human EGR1.
Epitope: Internal.

Anti-EGR1 Antibody, clone 16A10.1 is an antibody against EGR1 for use in Western Blotting, Immunocytochemistry.
Research Sub Category
Transcription Factors
Immunocytochemistry Analysis: An 1:250 dilution of this antibody from a representative lot detected EGF-induced upregulation of nuclear EGR1 immunoreactivity in serum-starved HeLa and NIH/3T3 cells.
Research Category
Apoptosis & Cancer

Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected VEGF-induced EGR1 expression in serum-starved HUVECs.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

16A10.1, monoclonal

human

immunocytochemistry: suitable
western blot: suitable

IgG1κ

NP_001955

P18146

wet ice

human ... EGR1(1958)

~80 kDa observed. 57.51 kDa calculated. The observed target band size is consistent with the reported apparent molecular weight (Krones-Herzig, A., et al. (2005). Cancer Res. 65(12):5133-5143; Josefsen, K., et al. (1999). Diabetologia. 42(2):195-203).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1