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Milli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE
产品编号: | 4096080 |
规格: | clone AW39R, Milli-Mark®, from rabbit |
包装规格: | 100 TESTS |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Milli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE
Erk1
Erk2
p44-MAPK
p42-MAPK
MAPK2
PRKM2
p41mapk
PRKM1
p44
ERK2
P42MAPK
ERK
p42
MAPK1
ERT1
ERT2
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively). |
Specificity【特异性】 | Antibody recognizes Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif. |
Immunogen【免疫原】 | Phosphorylated recombinant peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated Epitope: Phosphorylated Thr185/Phosphorylated Tyr187 |
Application【应用】 | Research Sub Category MAP Kinases This Milli-Mark Phospho-Erk1/2 antibody is validated for use in Flow & WB for the detection of the Milli-Mark Phospho-Erk1/2 protein. Research Category Signaling |
Quality【质量】 | Evaluated by Flow Cytometry with Jurkat cells. |
Physical form【外形】 | Purified Rabbit monoclonal supernatant conjugated to phycoerythrin in buffer containing PBS with 0.05% sodium azide Protein A purified |
Analysis Note【分析说明】 | Control Jurkat Cells |
Legal Information【法律信息】 | MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
conjugate【偶联物】 | PE |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | AW39R, monoclonal |
species reactivity | rat, mouse |
species reactivity (predicted by homology) | human (based on 100% sequence homology) |
manufacturer/tradename | Milli-Mark® |
technique(s) | flow cytometry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NP_002736.3 |
UniProt accession no.【UniProt登记号】 | P28482 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | 42kDa and 44kDa Observed |
Storage and Stability【储存及稳定性】 | Stable for 6 months at 2-8°C from date of receipt. Protect from light. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |