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Anti-NeuN purified Antibody
产品编号: | 4096041 |
规格: | from guinea pig, purified by affinity chromatography |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-NeuN purified Antibody
NEUronal Nuclei, clone A60, NeuN
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | ABN90P is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found. |
Specificity【特异性】 | This antibody recognizes the N-terminus of NeuN. |
Immunogen【免疫原】 | Epitope: N-terminus GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN. |
Application【应用】 | This Anti-NeuN purified Antibody is validated for use in Western Blotting, ICC, IHC(P) for the detection of NeuN purified. Research Sub Category Neuronal & Glial Markers Research Category Neuroscience Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NeuN in mouse frontal cortex tissue. Immunocytochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected NeuN in rat E18 cortex cells. |
Quality【质量】 | Evaluated by Western Blot in mouse E16 brain tissue lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected NeuN on 10 µg of mouse E16 brain tissue lysate. |
Physical form【外形】 | Purified guinea pig polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Antigen Affinity Purified |
Analysis Note【分析说明】 | Control Mouse E16 brain tissue lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | guinea pig |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | rat, mouse, human |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~48 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |