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Goat Anti-Rat light chain Antibody, HRP conjugate
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Light chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain′s mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species. |
Specificity【特异性】 | The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, mouse, human, rabbit, and sheep immunoglobulins. Minimal cross-reaction with bovine, goat, horse, mouse, human, rabbit, and sheep. |
Immunogen【免疫原】 | Prepared from purified rat IgG light chain. Epitope: Kappa light chain |
Application【应用】 | Research Sub Category Fragment Specific Secondary Antibodies Western Blotting: 1:5,000 -1:100,000 with chromogenic substrates 1:10,000 -1:200,000 with ECL substrates ELISA: 1:5,000 -1:100,000 Immunohistochemistry: 1:500 - 1:5,000 Immunocytochemistry: 1:500 - 1:5,000 Optimal working dilutions must be determined by the end user. Research Category Secondary & Control Antibodies Goat anti-Rat light chain Antibody, HRP conjugate is an antibody against light chain for use in ELISA, WB, IC, IH. |
Physical form【外形】 | Purified by immunoaffinity chromatography. Lyophilized in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. 15 mg/mL BSA as stabilizer. ImmunoAffinity Purified |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | goat |
conjugate【偶联物】 | peroxidase conjugate |
antibody form【抗体形式】 | affinity purified immunoglobulin |
antibody product type | secondary antibodies |
clone【克隆】 | polyclonal |
species reactivity | rat |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | 25 kDa |
Storage and Stability【储存及稳定性】 | Maintain lyophilized product at 2°-8°C for up to 12 months. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 3 |