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Goat Anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed
基本信息
eCl@ss | 32160702 |
NACRES | NA.46 |
General description【一般描述】 | Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
Application【应用】 | EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Optimal working dilutions must be determined by end user. Research Category Secondary & Control Antibodies Research Sub Category Secondary Antibodies Adsorbed for Dual Labeling This Goat anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Mouse IgG. |
Physical form【外形】 | Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA. RECONSTITUTION: Reconstitute with 500 μL of sterile distilled water. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | goat |
conjugate【偶联物】 | peroxidase conjugate |
antibody form【抗体形式】 | F(ab′)2 fragment of affinity isolated antibody |
antibody product type | secondary antibodies |
clone【克隆】 | polyclonal |
species reactivity | mouse |
manufacturer/tradename | Chemicon® |
concentration【浓度】 | 0.8 mg/mL |
technique(s) | ELISA: suitable western blot: suitable |
shipped in【运输】 | wet ice |
产品说明
Storage and Stability【储存及稳定性】 | Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
WGK | WGK 3 |