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Monoclonal Anti-p53-Biotin 小鼠抗
Monoclonal Anti-p53-Biotin antibody produced in mouse
产品编号: | 4095530 |
规格: | ~1 mg/mL, clone DO-1, ascites fluid, buffered aqueous solution |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Monoclonal Anti-p53-Biotin 小鼠抗
Monoclonal Anti-p53-Biotin antibody produced in mouse
基本信息
MDL number | MFCD01864735 |
NACRES | NA.41 |
General description【一般描述】 | p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11. |
Immunogen【免疫原】 | recombinant human wild type p53. The epitope resides beween amino acids 20-25 of human p53. |
Application【应用】 | Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting. |
Biochem/physiol Actions【生化/生理作用】 | Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung. |
Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
Other Notes【其他说明】 | In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration. |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | biotin conjugate |
antibody form【抗体形式】 | ascites fluid |
antibody product type | primary antibodies |
clone【克隆】 | DO-1, monoclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~53 kDa |
species reactivity | human |
concentration【浓度】 | ~1 mg/mL |
technique(s) | flow cytometry: suitable immunocytochemistry: 5-10 μg/mL using human A431 immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: 0.5-1 μg/mL using human A431 cell extracts |
isotype【同位素/亚型】 | IgG2a |
UniProt accession no.【UniProt登记号】 | P04637 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... TP53(7157) |
产品说明
Storage and Stability【储存及稳定性】 | For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree”freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |