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Anti-Podoplanin Antibody, clone LpMab-17

产品编号:4095119
规格:clone LpMab-17, from mouse
包装规格:25 μG,100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
409511925 μG1550
4095119100 μG5010
产品别名

Anti-Podoplanin Antibody, clone LpMab-17

Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-17 is reported to bind to non-PLAG domain and detects endogenous podoplanin in cancer cells and normal cells. It recognizes glycan-deficient podoplanin indicating that its interaction with podoplanin is independent of its glycosylation. The minimum epitope of LpMab-17 is identified as Gly77-Asp82. Although human and monkey podoplanin exhibit 94% homology, this clone does not recognize monkey podoplanin. (Ref.: Kato, Y., et al. (2016). Monoclon. Antib. immunodiag. Immunother. 35(2); 109-116).
Specificity【特异性】
Clone LpMab-17 detects podoplanin in human cells. It detects PLAG4 domain. The minimal epitope seunce is shown to be Gly77-Asp82.
Immunogen【免疫原】
LN229 glioma cells expressing human Podoplanin.
Application【应用】
Research Category
Cell Structure
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue.

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(2):109-16).

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(2):109-16).

Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(2):109-16).

ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(2):109-16).

Anti-Podoplanin, clone LpMab-17, Cat. No. MABT867, is a mouse monoclonal antibody that detects human podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality【质量】
Evaluated by Immunohistochemistry in human colon cancer tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in human colon cancer tissue.
Physical form【外形】
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
LpMab-17, monoclonal
species reactivity
human
packaging【包装】
antibody small pack of 25 μg
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NP_006465.3
UniProt accession no.【UniProt登记号】
Q86YL7
Gene Information
human ... PDPN(10630)
产品说明
Target description【目标描述】
16.7 kDa calculated.
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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