广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Anti-phospho-CaMKII beta (Ser331)
产品搜索:

Anti-phospho-CaMKII beta (Ser331)

产品编号:4095118
规格:from rabbit, purified by affinity chromatography
包装规格:25 μL,250 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
409511825 μL1550
4095118250 μL5010
产品别名

Anti-phospho-CaMKII beta (Ser331)

Calcium/calmodulin-dependent protein kinase type II subunit beta, EC: 2.7.11.17, CaM kinase II subunit beta, CaMK-II beta

基本信息
eCl@ss
32160702
General description【一般描述】
Calcium/calmodulin-dependent protein kinase type II subunit beta (UniProt: P08413; also known as EC: 2.7.11.17, CaM kinase II subunit beta, CaMK-II subunit beta) is encoded by the Camk2b gene (Gene ID: 24245) in rat. CaM kinase II is one of the most widely studied multi-functional enzyme. It is found in high concentrations in the brain and in some regions it may constitute up to 2% of the total protein content. CaM kinase II is a complex of about 12 subunits, which exist in four differentially expressed forms (alpha, beta, gamma, and delta). CaMKII beta contains an auto-inhibitory domain (aa 283-293), a calmodulin-binding domain (aa 291-301) and a unique C-terminal association domain responsible for oligomerization. It is activated Ca2+/calmodulin and binding of calmodulin results in conformational change that relieves intrasteric autoinhibition and allows autophosphorylation of Thr-287 which turns the kinase in a constitutively active form and confers to the kinase a Ca2+-independent activity that persists even after withdrawal of calcium. Phosphorylation of Thr-287 locks the kinase into an activated state. CaM Kinase II typically recognizes the phosphorylation site sequence Arg-X-X-Ser/Thr. It prefers arginine in the P3 position, which is reported to make an electrostatic contact with Glu96 in the catalytic core.
Specificity【特异性】
This rabbit polyclonal antibody detects beta subunit of CaM Kinase II when phosphorylated on Ser331.
Immunogen【免疫原】
A linear peptide corresponding to 10 amino acids surrounding phopho serine 331 of rat CaM Kinase II beta.
Application【应用】
Anti-phospho-CaMKII beta (Ser331), Cat. No. ABS2107, is a highly specific rabbit polyclonal antibody that targets Calcium/calmodulin-dependent protein kinase type II subunit beta (CaMK-II subunit beta) and has been tested for use in Immunocytochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT or mutants (Kim, K., et. al. (2015). Neuron. 87(4):813-26).

Immunocytochemistry Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in dissociated hippocampal neurons (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
Quality【质量】
Evaluated by Western Blotting in HEK293T cells transfected with CaMKII WT or mutant S331A.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT, but not the mutant S331A.
Other Notes【其他说明】
Affinity Purified
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
purified by【纯化方式】
affinity chromatography
species reactivity
rat
species reactivity (predicted by homology)
human (based on 100% sequence homology)
packaging【包装】
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NP_068507
UniProt accession no.【UniProt登记号】
P08413
产品说明
Target description【目标描述】
~60 kDa observed; 60.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.6113