Ret (V804L) Protein, active, 10 μg

32160405

NA.32

N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804L mutation.
The V804L mutation has been identified in patients with familial medullary thyroid carcinoma (FMTC) (Lombardo F. et al., (2002), JCEM, 87, 1674-1680; Lesueur F. et al, (2005), JCEM, 90, 3454-3457).
Product Source: Expressed by baculovirus in Sf21 insect cells.

Research Category
Inflammation & Immunology

Target Sub-Family: TK
Protein Target: Ret

Routinely evaluated by phosphorylation of CHKtide.

Glutathione agarose affinity chromatography
50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

300
100

human

M_w 79.2 kDa

Upstate^®

activity assay: suitable (kinase)

NM_000323

P07949
P35546 (mouse)

human ... RET(5979)

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-758M when ordering the 250 µg size.

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07

Warning

H317

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Skin Sens. 1

10 - Combustible liquids

WGK 1