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Interleukin-4 human
| 产品编号： | 4094025 |
| 规格： | IL-4, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested |
| 包装规格： | 50 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Interleukin-4 human
IL-4
基本信息
| MDL number | MFCD00151705 |
| NACRES | NA.77 |
| General description【一般描述】 | Recombinant human Interleukin-4 (IL-4) is expressed in human 293 cells as a glycoprotein with a calculated moleculaer mass of 14.9 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. |
| Biochem/physiol Actions【生化/生理作用】 | IL-4 is a protein that has been shown to regulate a wide spectrum of functions in B cells, T cells, monocytes/macrophages and other haematopoietic and non-haematopoietic cells. Among T cell clones, IL-4 is produced by Th0 and Th2 cells, but not Th1 cells, and this has now been demonstrated both in mice and in humans. IL-4 blocks the production of proinflammatory cytokines such IL-6, TNFα, and M-CSF and improves the differentiation of immature DCs from CD34+ progenitors when added during the differentiation period (day 6 to day 12). Interleukin-4 is a lymphokine with profound effects on the growth and differentiation of immunologically competent cells. IL-4 is also known as B cell stimulatory factor-1 (BSF-1), T cell growth factor-2 (TCGF-2) and mast-cell growth factor-2 (MCGF-2). Inhibits VEGF-induced and bFGF-induced angiogenesis. IL-4 is a complex glycoprotein released by a subset of activated T cells. Human and mouse IL-4 share 50% amino acid sequence homology, but their biological actions are species-specific. |
| Sequence【序列】 | HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS |
| Physical form【外形】 | Supplied as a lyophilized powder containing phosphate buffered saline. |
| Analysis Note【分析说明】 | The biological activity of recombinant human IL-4 was tested in culture by measuring its ability to stimulate proliferation of human TF-1 cells (human erythroleukemic indicator cell line). |
产品性质
| recombinant【重组】 | expressed in HEK 293 cells |
| Quality Level【质量水平】 | 200 |
| Assay【测定】 | ≥98% (SDS-PAGE) |
| potency【效能】 | ≤1 ng/mL ED50 |
| mol wt【分子量】 | 14.9 kDa (glycosylated) |
| technique(s) | cell culture | mammalian: suitable |
| suitability【适用性】 | endotoxin tested |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Hazard Statements | H412 |
| Precautionary Statements | P273 - P501 |
| Hazard Classifications【危险分类】 | Aquatic Chronic 3 |
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
