Anti-dimethyl-Histone H4 (Arg3) Asymmetric Antibody

32160702

NA.41

Histone H4 has multiple isoform names including H4/A through H4/N, and is encoded by multiple genes in the genome named HIST1H4A through HIST1H4N/H4/A or H4FA through H4/L and HIST2H4A, HIST2H4B, and HIST4H4. Histone H4 is a critical component of chromatin and the nucleosome. Histone H4 forms a multimeric cluster with the other histone members to regulate chromatin and DNA structure within the nucleus. Histone H4 is a target of much post-translational modification including acetylation, methylation, phosphorylation, ubiquitination, sumoylation, crotonylation (male germ cells only and marks testis specific genes) and citrullination. Acetylation of histone H4 on lysine 16 (H4-K16Ac) is the most prevalent and reversible posttranslational chromatin modification in eukaryotes for histone H4 and it and the other modifications help control chromatin structure and the protein interactions of histone H4.

KLH-conjugated linear peptide corresponding to the N-terminus of human dimethyl-Histone H4 (Arg3) Asymmetric.
Epitope: N-terminus

This Anti-dimethyl-Histone H4 (Arg3) Asymmetric Antibody is validated for use in Western Blotting and Immunoprecipitation and Dot Blot for the detection of dimethyl-Histone H4 (Arg3) Asymmetric.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Dot Blot Specificity Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with dimethyl-Histone H4 (Arg3) Asymmetric (1:2,000 dilution).
Immunoprecipitation Analysis: A 1:3,333 dilution from a representative lot immunoprecipitated dimethyl-Histone H4 (Arg3) Asymmetric in 500 µg of HeLa and 500ng of HeLa AE cell lysate.

Evaluated by Western Blotting of Histone H4 recombinant protein.

Western Blotting Analysis: A 1:3,333 dilution of this antibody detected dimethyl-Histone H4 (Arg3) Asymmetric in 500 ng of PRMT1 treated Histone H4 recombinant protein.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Concentration: Please refer to lot specific datasheet.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

mouse, rat, human

dot blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_001029249

P62805

wet ice

human ... HIST2H4B(554313)

~13 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1