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Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody

产品编号:4088554
规格:serum, from rabbit
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4088554100 μL5690
产品别名

Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody

Sodium/glucose cotransporter 1, Solute carrier family 5 member 1, Solute carrier family 5 (sodium/glucose cotransporter), member 1, Solute carrier family 5 (sodium/glucose transporter),member 1

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
The kidneys play a major role in the regulation of blood glucose. Although kidney nephrons indiscriminatly filter about 180g of glucose per day from the blood, most is reabsorbed actively in the proximal convoluted tubule by a Na+/Gl co-transport (ratio 2:1) mechanism (SGLT). Typically, glucose is first reabsorbed by these transporters, which reside within the brush-border epithelia of the PCT cells. The glucose is then transported out of the cell across the basolateral membranes by a facilitated diffusion sugar transporter (GLUTs). At least three members of SGLT′s (SGLT1-3) have been cloned and characterized from various species. SGLT1-3 genes code for protein of approximately 659-672 residues, with both N and C termini predicted to be extracellular. There is approximately 60-70% homology between the 3 forms. SGLT1 does not discriminate a-MDG, glucose and galactose, while SGLT2 and 3 do not transport D-galactose efficiently. SGLT1 is an integral membrane protein expressed in proximal convoluted tubule Sq1 segments of the kidney (outer renal medulla) and in the intestine.
Specificity【特异性】
Recognizes SGLT-1.
Immunogen【免疫原】
Synthetic peptide corresponding to amino acids 402-420 in the cytoplasmic region of SGLT-1 of mouse/rabbit small intestine.
Application【应用】
Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody detects level of SGLT-1 (Sodium Glucose Co-transporter-1) & has been published & validated for use in WB, IH(P), ELISA.
Research Category
Signaling
Immunohistochemisty (paraffin): Anti-SGLT-1 staining on human kidney, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was used at 1 μg/mL, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the brush boarder of proximal convoluted tubule.

ELISA: 1:10,000 - 1:50,000 dilution of a previous lot, using 50-100 ng of control peptide/well.

Optimal working dilutions must be determined by end user.

Western Blot: A previous lot of this antibody was used on multiple lysates. See datasheet.
Research Sub Category
Insulin/Energy Signaling
Quality【质量】
Routinely evaluated by Western Blot on PC3 lysates.

Western Blot Analysis:
1:500-1:2,000 dilution of this lot detected SGLT-1 on 10 μg of PC3 lysates.
Linkage【联系】
Replaces: AB1352
Physical form【外形】
Rabbit polyclonal IgG antiserum in buffer containing 0.1% sodium azide.
Analysis Note【分析说明】
Control
PC3 cell lysate
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
serum
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
feline, pig, rat, avian, human, rabbit, canine, reptile, mouse
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NM_000343.1
UniProt accession no.【UniProt登记号】
Q8C3K6
shipped in【运输】
dry ice
Gene Information
human ... SLC5A1(6523)
产品说明
Target description【目标描述】
Recognizes a band of approximately 70-77 kDa in Western blot.
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt in undiluted aliquots.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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