Anti-PDGF-BB Antibody

32160702

NA.41

Platelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin.

Reacts with human PDGF-BB.

Highly purified (>98%) recombinant human PDGF-BB

Research Sub Category
Growth Factors & Receptors
Research Category
Signaling
ELISA: 0.5 μg/mL dilution of a previous lot will allow detection (in direct ELISA) of
0.2-0.4 ng/well when human PDGF-BB is used to coat the plate.

Neutralization: To yield ½ maximal inhibition (ND50) of the biological activity of hPDGF-BB (2.5
ng/mL), a concentration of 0.03-0.04 μg/mL of a previous lot of this antibody was required.

Immunohistochemistry(paraffin): Anti-IGF-1R, representative staining-pattern and morphology on ductal breast carcinoma. Tissue was pretreated with EDTA, pH 8.0 for Epitope retrieval. A previous lot of this antibody was diluted to 1:200 (RT incubation), using IHC-Select Detection with HRP-DAB.

Optimal working dilutions must be determined by the end user.
Anti-PDGF-BB Antibody detects level of PDGF-BB & has been published & validated for use in WB, ELISA, NEUT.

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis:
1:500 dilution of this lot detected PDGF-BB on 10 μg of Huvec lysates.

Replaces: AB1487P

Purified rabbit polyclonal IgG lyophilized from 0.5X PBS, pH 7.4. Reconstitute with sterile distilled water.
Affinity purified

rabbit

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

human

ELISA: suitable
neutralization: suitable
western blot: suitable

IgG

NM_002608

P01127

dry ice

human ... PDGFB(5155)

28 kDa

Maintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

13 - Non Combustible Solids

WGK 1