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Anti-Bak Antibody, NT
产品别名
Anti-Bak Antibody, NT
Apoptosis regulator BAK, BCL2-antagonist/killer 1, BCL2-like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like 7 protein, pro-apoptotic protein BAK
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress. |
Specificity【特异性】 | Specific for Bak, MW ~28 kDa. |
Immunogen【免疫原】 | Synthetic peptide (SEEQVAQQDTEEVFRSC) corresponding to amino acid residues 23-38 of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH. Epitope: N-terminus |
Application【应用】 | Research Sub Category BCL2 & Inhibition Detect Bak using this Anti-Bak Antibody, NT validated for use in IC, IH, IP & WB. Research Category Apoptosis & Cancer Immunohistochemistry (Paraffin): 5 μg/mL of a previous lot detected Bak in paraffin-embedded rat kidney tissue. Immunoprecipitation: 4 μg of a previous lot immunoprecipitated Bak from 500 μg mouse 3T3 cell lysate. |
Quality【质量】 | Evaluated by Western Blot on HEK293 lysates. Western Blotting Analysis: 1:500 dilution of this antibody detected Bak on 10 µg of HEK293 lysates. |
Linkage【联系】 | Replaces: 04-433 |
Physical form【外形】 | Format: Purified Protein A purified Purified rabbit polyclonal in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen liquid. |
Analysis Note【分析说明】 | Control Included Positive Antigen Control: Catalog # 12-301, non-stimulated human A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | mouse, human, rat |
manufacturer/tradename | Upstate® |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NM_001188 |
UniProt accession no.【UniProt登记号】 | Q16611 |
shipped in【运输】 | dry ice |
Gene Information | human ... BAK1(578) |
产品说明
Target description【目标描述】 | 28 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20ºC from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |