您的位置:首页 > 产品中心 > Anti-Histone H3 Antibody
Anti-Histone H3 Antibody
产品别名
Anti-Histone H3 Antibody
Histone H3.1t, H3/t, H3t, H3/g
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Histone H3, also known as Histone H3.1t, H3/t, H3t, or H3/g, and encoded by the gene HIST3H3/H3Ft, is one of the core components of the nucleosome. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The octamer wraps approximately 147 bp of DNA. A classic nuclear marker, new expression is seen during S phase of the cell cycle. Histone H3 is often acetylated and or methylated multiple times. Changes in methylation or acetylation state of histone H3 together with similar modification on the sister histones is one key aspect of epigenetic gene expression and regulation. |
Specificity【特异性】 | This antibody recognizes Histone H3 at the N-terminus. Broad species cross-reactivity is expected based on sequence similarity. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3. Epitope: N-terminus |
Application【应用】 | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Histone H3 in HeLa cells. Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Histone H3 from 500 µg of HeLa acid extract. Immunoprecipitated sample was then subjected to Western Blotting using 0.5 µg/mL of the same antibody. Chromatin Immunoprecipitaton: A representative lot from an independent laboratory immunoprecipitated Histone H3 from plant chromatin (Ng, D. W., et al. (2006). Plant Cell. 18(1):119-132.). Research Sub Category Histones Anti-Histone H3 Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 also known as histone 3-H3 & has been validated in ChIP, ICC & WB. Research Category Epigenetics & Nuclear Function |
Quality【质量】 | Evaluated by Western Blotting in HeLa acid extract. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 in 10 µg of HeLa acid extract. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control HeLa acid extract |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human, rat, mouse |
manufacturer/tradename | Upstate® |
concentration【浓度】 | 0.5 mg/mL |
technique(s) | ChIP: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_003484 |
UniProt accession no.【UniProt登记号】 | Q16695 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~17 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |