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Anti-Sonic Hedgehog Antibody

产品编号:4088129
规格:from rabbit, purified by affinity chromatography
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4088129100 μL5010
产品别名

Anti-Sonic Hedgehog Antibody

sonic hedgehog, sonic hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila)

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal. Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene. Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo. A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma.
Specificity【特异性】
This antibody recognizes the N-terminus of Sonic Hedgehog.
Immunogen【免疫原】
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of Sonic HedgeHog.
Application【应用】
Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate.

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Sonic Hedgehog in colorectal carcinoma tissue.
Anti-Sonic Hedgehog Antibody detects level of Sonic Hedgehog & has been published & validated for use in WB, IH.
Research Category
Signaling
Research Sub Category
Developmental Signaling
Quality【质量】
Evaluated by Western Blot in human fetal skeletal muscle tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate.
Linkage【联系】
Replaces: 04-971
Physical form【外形】
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified
Analysis Note【分析说明】
Control
Human fetal skeletal muscle tissue lysate
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
purified by【纯化方式】
affinity chromatography
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.【NCBI登记号】
NP_000184
UniProt accession no.【UniProt登记号】
Q15465
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
~ 45 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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