您的位置:首页 > 产品中心 > Anti-Phosphotyrosine Antibody
Anti-Phosphotyrosine Antibody
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function. |
Specificity【特异性】 | The immunoreactivity of the antibody is totally inhibited by the use of 100 mM phenyl phosphate, a phosphotyrosine analog. The phosphotyrosine antibody is purified by immunoaffinity chromatography using either a dual phospho-peptide gel or a BSA-phosphotyrosine gel. All of the phosphotyrosine immunoreactivity present in the antisera is immunoadsorbed whether the antibody is purified by either gel indicating that the antibody is not sequence-specific but specific for phosphotyrosine residues. |
Immunogen【免疫原】 | In order to produce broad spectrum polyclonal phosphotyrosine antibodies, rabbits were immunized with three phosphorylated immunogens: (1) phosphotyrosine covalently linked to KLH; (2) the c-Src carboxyl terminal regulatory phosphopeptide (TS-T-E-P-Q-pY-Q-P-G-E-N-L; Catalog # 12-218) covalently linked to KLH, and; (3) a phosphopeptide associated with high tyrosine kinase activity in human lymphocytes (R-R-L-I-E-D-A-E-pY-A-A-R-G; Catalog # 12-217) covalently linked to KLH. Both of the phosphopeptide haptens serve as strong substrates for tyrosine phosphatases and are part of the two colorimetric tyrosine phosphatase kits provided by Upstate, Inc. (Catalog # 17-125, 17-126). |
Application【应用】 | Western Blotting Analysis: A 1:500 dilution from a representative lot detected Phosphotyrosine in 10 µg of EGF treated A431 with Lambda Phosphatase tissue lysate. Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated Phosphotyrosine in 0.5 mg of EGF treated A431 cell lysate Research Category Signaling Anti-Phosphotyrosine Antibody detects level of Phosphotyrosine & has been published & validated for use in IP & WB. Research Sub Category General Post-translation Modification Signaling Neuroscience |
Quality【质量】 | Evaluated by Western Blotting in EGF treated A431 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Phosphotyrosine in 10 µg of EGF treated A431 cell lysate. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | all (based on 100% sequence homology) |
manufacturer/tradename | Upstate® |
technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | Varies |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |