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Anti-NFκB p52 Antibody
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | The NFκB transcription factor was originally identified as a protein complex consisting of a 65 kDa DNA binding subunit and an associated 50 kDa protein. The 65 kDa subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a 100 kDa protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells. |
Specificity【特异性】 | Recognizes NFκB p52 and its precursor. |
Immunogen【免疫原】 | KLH conjugated synthetic peptide (CYNPGLDGIIEYDDFK) contained within amino acids 4-19 of human NF κB, p52 |
Application【应用】 | Research Sub Category Transcription Factors Detect NFκB p52 with Anti-NFκB p52 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB. Research Category Epigenetics & Nuclear Function |
Quality【质量】 | routinely evaluated by immunoblot on RIPA lysates from Raji cells |
Physical form【外形】 | Unpurified Format: Purified Protein A Purified immunoglobulin in in 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% NaN3 as a preservative. |
Analysis Note【分析说明】 | Control Raji cell lysate, Jurkat whole cell lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | using Protein A |
species reactivity | human |
manufacturer/tradename | Upstate® |
technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NM_019408 |
UniProt accession no.【UniProt登记号】 | Q00653 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | 52 kDa, and precursor (100 kDa) |
Storage and Stability【储存及稳定性】 | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |