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Anti-GFP (Green Fluorescent Protein) Antibody
产品别名
Anti-GFP (Green Fluorescent Protein) Antibody
Green Fluorescent Protein
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Green fluorescent protein (GFP) is a 29 kDa fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488 nm), GFP strongly fluoresces (Emission max: 507 nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells. |
Specificity【特异性】 | Recognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins. |
Immunogen【免疫原】 | His-tagged green fluorescent protein of Aequorea victoria. |
Application【应用】 | Research Category Epitope Tags & General Use Research Sub Category Epitope Tags Immunocytochemistry: This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein. Anti-GFP (Green Fluorescent Protein) Antibody detects level of GFP (Green Fluorescent Protein) & has been published & validated for use in IC & WB. |
Quality【质量】 | Routinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate. Western Blot Analysis: 0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate. |
Physical form【外形】 | Purified chicken polyclonal IgY from egg yolk in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C. Ammonium Sulfate Precipitation and PEG Format: Purified |
Analysis Note【分析说明】 | Control Rat brain lysate, cells expressing GFP protein. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | chicken |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | vertebrates |
manufacturer/tradename | Upstate® |
technique(s) | immunocytochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgY |
UniProt accession no.【UniProt登记号】 | P42212 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | 30 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |