Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1

32160702

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Tyrosine Hydroxylase. Recognizes a protein of approximately 59-63 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase, phenethanolamine-N-methyl transferase (PNMT).

Tyrosine Hydroxylase purified from PC12 cells.
Epitope: Recognizes an epitope on the outside of the regulatory N-terminus of tyrosine hydroxylase.

Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1 detects level of Tyrosine Hydroxylase & has been published & validated for use in IH(P).
Research Sub Category
Neuronal & Glial Markers
Research Category
Neuroscience
Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 30 minutes with IHC Select Detection Kits.

Tyrosine Hydroxylase has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR1005-6.

Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.
Format: Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified antibody

primary antibodies

LNC1, monoclonal

monkey, frog, mouse, chicken, lizard, vole, human, rat, zebrafish

Chemicon^®

immunohistochemistry: suitable (paraffin)

IgG1κ

NM_000360.3
NM_199292.2
NM_199293.2

P07101

wet ice

Maintain at 2-8°C. Refer to vial for expiration dating.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07

Warning

H317 - H412

P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313

Aquatic Chronic 3 - Skin Sens. 1

12 - Non Combustible Liquids

WGK 2