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Anti-Choline Acetyltransferase (ChAT) Antibody
产品别名
Anti-Choline Acetyltransferase (ChAT) Antibody
Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker. |
Specificity【特异性】 | Reactivity with other species has not been confirmed with IHC. Cholinergic neurons in brain and central nervous systems. |
Application【应用】 | Anti-Choline Acetyltransferase (ChAT) Antibody is an antibody against Choline Acetyltransferase (ChAT) for use in IH, IH(P), IP & WB with more than 70 product citations. Immunoprecipitation: 1:200 dilution of a previous lot immunoprecipitated 68 kDa active ChAT from brain and placenta. Also precipitates a 27 kDa protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of Humans and possibly other mammals. Optimal working dilutions and protocls must be determined by end user. |
Quality【质量】 | Routinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue. Immunohistochemistry (Paraffin) Analysis: ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select |
Physical form【外形】 | Unpurified Rabbit polyclonal antisera, liquid, no preservatives. |
Analysis Note【分析说明】 | Control Cerebral cortex tissue. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | monkey, mouse, feline, rat, bat, human |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_065574 |
UniProt accession no.【UniProt登记号】 | P28329 |
shipped in【运输】 | dry ice |
产品说明
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |