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Anti-acetyl Histone H3 Antibody (Lys122)
产品编号: | 4087676 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-acetyl Histone H3 Antibody (Lys122)
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. |
Immunogen【免疫原】 | Epitope: Near C-terminal KLH-conjugated linear peptide corresponding to human acetyl Histone H3 (Lys122) near the C-terminal. |
Application【应用】 | Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected acetyl Histone H3 (Lys122) in HeLa and A431cells. Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl Histone H3 (Lys122) in Histone peptides with various modifications. Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated acetyl Histone H3 (Lys122) in 500 µg of sodium butyrate treated HeLa acid extract. Research Sub Category Nuclear Receptors Research Category Epigenetics & Nuclear Function Anti-acetyl Histone H3 (Lys122) is an antibody against acetyl Histone H3 (Lys122) for use in WB, ICC, DB, IP. |
Quality【质量】 | Evaluated by Western Blotting in sodium butyrate treated HeLa acid extract. Western Blotting Analysis: 1.0 µg/mL of this antibody detected acetyl Histone H3 (Lys122) in 10 µg of sodium butyrate treated HeLa acid extract. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | mouse, human |
technique(s) | dot blot: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_003520 |
UniProt accession no.【UniProt登记号】 | P68431 |
shipped in【运输】 | wet ice |
Gene Information | human ... HIST1H3F(8968) |
产品说明
Target description【目标描述】 | ~17 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |