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PhosphoDetect Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb

产品编号:4087664
规格:liquid, Calbiochem®
包装规格:10 TESTS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
408766410 TESTS7530
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
form【形式】
liquid
contains【包含】
≤0.1% sodium azide as preservative
species reactivity
rat, mouse, human
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
avoid repeated freeze/thaw cycles
isotype【同位素/亚型】
IgG
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
packaging【包装】
10 tests in Plastic ampoule
基本信息
General description【一般描述】
Recognizes c-Raf phosphorylated at Tyr340 and Tyr341 in HEK293 cells overexpressing c-Raf.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr340/341.
This PhosphoDetect Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb is validated for use in WB, ICC for the detection of c-Raf (pTyr³⁴⁰, Tyr³⁴¹).
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Immunogen【免疫原】
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
Human
Application【应用】
Immunoblotting (1:1000)

Immunocytochemistry (see comments)
Warning【警告】
Toxicity: Standard Handling (A)
Physical form【外形】
In phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).
Reconstitution【重悬】
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note【分析说明】
Positive Control
HEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.
Other Notes【其他说明】
The immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Yan, F., and Polk, D.B. 2001. Cancer Res.61, 963.
Yip-Schneider, M.T., et al. 2000. Biochem. J.351, 151.
Mason, C.S. et al. 1999. EMBO J.18, 2137.
Bonner, T.I. et al. 1985. Mol. Cell. Biol.5, 1400.
Moelling, K. et al. 1984. Nature312, 558.
Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA80, 4218.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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