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PhosphoDetect Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity | rat, mouse, human |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
isotype【同位素/亚型】 | IgG |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 10 tests in Plastic ampoule |
基本信息
General description【一般描述】 | Recognizes c-Raf phosphorylated at Tyr340 and Tyr341 in HEK293 cells overexpressing c-Raf. Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr340/341. This PhosphoDetect Note: 1 T = 1 test. Sufficient for 10 Western miniblots. |
Immunogen【免疫原】 | a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1) Human |
Application【应用】 | Immunoblotting (1:1000) Immunocytochemistry (see comments) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1). |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
Analysis Note【分析说明】 | Positive Control HEK293 cells over expressing c-Raf, phosphorylated in vitro by Src. |
Other Notes【其他说明】 | The immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution. Yan, F., and Polk, D.B. 2001. Cancer Res.61, 963. Yip-Schneider, M.T., et al. 2000. Biochem. J.351, 151. Mason, C.S. et al. 1999. EMBO J.18, 2137. Bonner, T.I. et al. 1985. Mol. Cell. Biol.5, 1400. Moelling, K. et al. 1984. Nature312, 558. Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA80, 4218. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |