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Anti-NQO1 (C-terminal) 兔抗
Anti-NQO1 (C-terminal) antibody produced in rabbit
产品编号: | 4087659 |
规格: | ~1 mg/mL, affinity isolated antibody, buffered aqueous solution |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-NQO1 (C-terminal) 兔抗
Anti-NQO1 (C-terminal) antibody produced in rabbit
Anti-DT-diaphorase
Anti-NAD (P)H:Menadione oxidoreductase 1, dioxin-inducible 1
Anti-NAD(P)H dehydrogenase, quinone 1
Anti-Diaphorase 4
Anti-NMOR1
Anti-DIA4
基本信息
NACRES | NA.41 |
General description【一般描述】 | NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) is a 2-electron reductase. NQO1 is a FAD-containing protein that exists as a homodimer. It is ubiquitously expressed. NADPH:quinone oxidoreductase (NQO1) is an FAD containing electron reductase that regulates the detoxification of quinones obtained from the phenolic metabolites of benzene. It is a homodimeric protein that can bind either of the reducing cofactors; NADPH or NADH . NQO1 regulates chemoprotection, neuroprotection, antitumor quinones and antioxidative defense mechanisms . NQO1 may be overexpressed in breast, colon, pulmonary and hepatic tumors. |
Specificity【特异性】 | Anti-NQO1 (C-terminal) antibody is specific for rat, mouse, and human NQO1. The immunizing peptide specifically inhibits the detection of NQO1 band by immunoblotting. |
Immunogen【免疫原】 | Synthetic peptide sequence corresponding to amino acid residues 261-274 of human NQO1, with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat. synthetic peptide corresponding to amino acid resudues 261-274 of human NQO1 with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat. |
Application【应用】 | Anti-NQO1 (C-terminal) antibody produced in rabbit has been used in:
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) Anti-NQO1 (C-terminal) antibody may be used in immunological applications such as immunoprecipitation, immunofluorescence and immunoblotting (approximately 28 kDa). |
Biochem/physiol Actions【生化/生理作用】 | NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) can bioactivate certain antitumor quinones. NQO1 has a role in antioxidant defense via the generation of antioxidant forms of ubiquinone and vitamin E. High levels of NQO1 gene expression has been observed in liver and lung tumors as compared to normal tissues of the same origin. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~28 kDa |
species reactivity | mouse, human, rat |
concentration【浓度】 | ~1 mg/mL |
technique(s) | immunoprecipitation (IP): 2-5 μg using whole extract of human HeLa cells indirect immunofluorescence: 2-5 μg/mL using human HeLa cells western blot: 0.1-0.2 μg/mL using whole extract of rat NRK cells western blot: 0.2-0.5 μg/mL using whole extract of mouse 3T3 cells |
UniProt accession no.【UniProt登记号】 | P15559 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... NQO1(1728) mouse ... Nqo1(18104) rat ... Nqo1(24314) |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |