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Anti-phospho-CYLD Antibody (Ser418)
产品别名
Anti-phospho-CYLD Antibody (Ser418)
Ubiquitin carboxyl-terminal hydrolase CYLD, Deubiquitinating enzyme CYLD, Ubiquitin thioesterase CYLD, Ubiquitin-specific-processing protease CYLD
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | CYLD is a deubiquitinating enzyme that inactivates transcription factor NF-kappaB using TNFRs and is essential for normal cellular homeostasis of skin appendages. Deactivation or mutation of CYLD causes a loss of the deubiquitination activity and increases tumorigenesis. Loss of CYLD specifically results in familial cylindromatosis which is characterized by a predisposition to cylindromas, tumors of skin appendages. CYLD has been shown to cleave the lysine 63-linked polyubiquitin chain from proteins and the inactivity of CYLD has also been noticed in many other human cancers. |
Specificity【特异性】 | This antibody detects phospho-CYLD at Ser418. The phosphorylation site of CYLD shares some sequence homology with serine phosphorylation site of c-Cbl. |
Immunogen【免疫原】 | Linear peptide corresponding to human phospho-CYLD (Ser418). |
Application【应用】 | Research Sub Category Apoptosis - Additional Research Category Apoptosis & Cancer Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-CYLD (Ser418) in 10 µg of human spleen and Jurkat lysate. Western Blotting Analysis: A representative lot detected phospho-CYLD (Ser418) in PMA and Ionomycin-treated Jurkat cell lysate (Reiley, W., et al. (2005). Mole. Cell Biol. 25(10):3886-3895). This Anti-phospho-CYLD Antibody (Ser418) is validated for use in Western Blotting for the detection of phospho-CYLD (Ser418). |
Quality【质量】 | Evaluated by Western Blotting in Raji cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-CYLD (Ser418) in 10 µg of Raji cell lysate. |
Physical form【外形】 | Rabbit polyclonal serum with 0.05% sodium azide. Unpurified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | human |
technique(s) | western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_001035814 |
UniProt accession no.【UniProt登记号】 | Q9NQC7 |
shipped in【运输】 | wet ice |
Gene Information | human ... CYLD(1540) |
产品说明
Target description【目标描述】 | ~110 kDa observed. Uncharacterized band(s) may appear in some lysates. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |