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Anti-Nestin, Human Antibody

产品编号:4087640
规格:serum, from rabbit
包装规格:50 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
408764050 μL6020
产品别名

Anti-Nestin, Human Antibody

Nestin

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
Specificity【特异性】
This antibody recognizes human Nestin. Antibody is not reactive with rat and mouse Nestin protein.
Immunogen【免疫原】
GST-tagged recombinant protein corresponding to human Nestin.
Application【应用】
Evaluated by Immunocytochemistry in human WI38 cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.
Detect Nestin, Human using this Anti-Nestin, Human Antibody validated for use in Western Blotting, ICC & IHC.
Quality【质量】
Evaluated by Immunocytochemistry in human U251 glioblastoma cells. Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human U251 glioblastoma cells.
Linkage【联系】
Replaces: AB5922
Analysis Note【分析说明】
Unpurified
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
serum
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
human
should not react with
mouse, rat
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.【NCBI登记号】
NP_006608
UniProt accession no.【UniProt登记号】
P48681
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
The anti-Nestin antibody has an observed MW at 220kDa Antibody may see 260kDa band in some cells and/or treatments
【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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