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Anti-Histone Macro H2A.1 Antibody
产品编号: | 4087637 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-Histone Macro H2A.1 Antibody
Core histone macro-H2A.1, Histone macroH2A1.2, mH2A1, H2A.y, H2A/y
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Histone MacroH2A (mH2A) is a variant histone also known as H2AFY. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. This histone variant is known to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. In contrast to most histone variants containing only subtle changes, mH2A contains a large non-histone domain (the macro domain) on its C-terminus, resulting in a histone approximately three times the size of conventional H2A. There are 3 isoforms of mH2A, two of which (mH2A1.1, mH2A1.2 ) are formed by alternative splicing and another (mH2A2) from a separate gene. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of posttranslational modifications throughout the cell cycle. |
Specificity【特异性】 | This antibody recognizes Histone MacroH2A.1 at the macro domain. |
Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to mcro domain of mouse Histone MacroH2A.1. Epitope: Macro domain |
Application【应用】 | Research Sub Category Histones Research Category Epigenetics & Nuclear Function Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone MacroH2A.1 in NIH/3T3, A431, and HeLa cells. Anti-Histone Macro H2A.1 Antibody is a Rabbit Polyclonal Antibody for detection of Histone Macro H2A.1 also known as H2A histone family member Y, histone macroH2A1.2 & has been validated in WB, ICC. |
Quality【质量】 | Evaluated by Western Blot in HeLa acid extract. Western Blot Analysis: 0.2 µg/mL of this antibody detected histone MacroH2A.1 on 10 µg of HeLa acid extract. |
Linkage【联系】 | Replaces: 07-219 |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control HeLa acid extract |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | mouse, human |
technique(s) | immunocytochemistry: suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_001152985.1 |
UniProt accession no.【UniProt登记号】 | Q9QZQ8 |
shipped in【运输】 | wet ice |
Gene Information | human ... H2AFJ(55766) |
产品说明
Target description【目标描述】 | ~ 40 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |