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Anti-phospho TIE2/TEK Antibody (Tyr992)
产品别名
Anti-phospho TIE2/TEK Antibody (Tyr992)
Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, hTIE2, p140 TEK, CD202b
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | TIE2/TEK (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2/TEK are associated with inherited venous malformations; the TIE2/TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to the Protein Kinase Domain of human TIE2/TEK phosphorylated at Tyr992. Epitope: Protein Kinase Domain |
Application【应用】 | Research Sub Category Inflammation & Autoimmune Mechanisms This Anti-phospho TIE2/TEK Antibody (Tyr992) is validated for use in western blotting, IP & peptide inhibition assay for the detection of phospho TIE2/TEK (Tyr992). Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated phospho TIE2/TEK (Tyr992) from Pervanadate treated HUVEC cell lysate. Immunoprecipated sample was then subjected to Western Blotting using a 1:5,000 dilution of this antibody. Peptide Inhibition Assay Analysis: A 1:500 dilution from a representative lot detected phospho TIE2/TEK (Tyr992) from transfected HEK293T lysates when pre-incubated without or with non-phospho peptide, and not when pre-incubated with phospho peptide. Research Category Inflammation & Immunology |
Quality【质量】 | Evaluated by Western Blotting in untreated and Pervanadate treated HUVEC cell lysate. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho TIE2/TEK (Tyr992) in 10 µg of Pervanadate treated HUVEC cell lysate, and not in untreated HUVEC cell lysate. |
Linkage【联系】 | Replaces: 09-407 |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology) |
concentration【浓度】 | 0.25 mg/mL |
technique(s) | immunoprecipitation (IP): suitable inhibition assay: suitable (peptide) western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_000450 |
UniProt accession no.【UniProt登记号】 | Q02763 |
shipped in【运输】 | wet ice |
Gene Information | human ... TEK(7010) |
产品说明
Target description【目标描述】 | ~125 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |