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Anti-phospho TIE2/TEK Antibody (Tyr992)

产品编号:4087629
规格:0.25 mg/mL, from rabbit
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4087629100 μL6020
产品别名

Anti-phospho TIE2/TEK Antibody (Tyr992)

Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, hTIE2, p140 TEK, CD202b

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
TIE2/TEK (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2/TEK are associated with inherited venous malformations; the TIE2/TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
Immunogen【免疫原】
KLH-conjugated linear peptide corresponding to the Protein Kinase Domain of human TIE2/TEK phosphorylated at Tyr992.
Epitope: Protein Kinase Domain
Application【应用】
Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-phospho TIE2/TEK Antibody (Tyr992) is validated for use in western blotting, IP & peptide inhibition assay for the detection of phospho TIE2/TEK (Tyr992).
Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated phospho TIE2/TEK (Tyr992) from Pervanadate treated HUVEC cell lysate. Immunoprecipated sample was then subjected to Western Blotting using a 1:5,000 dilution of this antibody.

Peptide Inhibition Assay Analysis: A 1:500 dilution from a representative lot detected phospho TIE2/TEK (Tyr992) from transfected HEK293T lysates when pre-incubated without or with non-phospho peptide, and not when pre-incubated with phospho peptide.
Research Category
Inflammation & Immunology
Quality【质量】
Evaluated by Western Blotting in untreated and Pervanadate treated HUVEC cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho TIE2/TEK (Tyr992) in 10 µg of Pervanadate treated HUVEC cell lysate, and not in untreated HUVEC cell lysate.
Linkage【联系】
Replaces: 09-407
Physical form【外形】
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
purified by【纯化方式】
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
concentration【浓度】
0.25 mg/mL
technique(s)
immunoprecipitation (IP): suitable
inhibition assay: suitable (peptide)
western blot: suitable
NCBI accession no.【NCBI登记号】
NP_000450
UniProt accession no.【UniProt登记号】
Q02763
shipped in【运输】
wet ice
Gene Information
human ... TEK(7010)
产品说明
Target description【目标描述】
~125 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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