您的位置:首页 > 产品中心 > Anti-Transcription Initiation Factor IIA subunit 1
Anti-Transcription Initiation Factor IIA subunit 1
产品编号: | 4087615 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 25 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-Transcription Initiation Factor IIA subunit 1
General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42
基本信息
eCl@ss | 32160702 |
General description【一般描述】 | Transcription initiation factor IIA subunit 1 (UniProt: P52655; also known as General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42) is encoded by the GTF2A1 (also known as TF2A1) gene (Gene ID: 2957) in human. TFIIA is one of the several components of the transcription machinery of RNA polymerase II that plays an important role in transcriptional activation. TFIIA in a complex with TATA-box-binding protein (TBP) mediates transcriptional activity. After initiation, some components of the pre-initiation complex, including TFIIA, remain attached and re-initiate a subsequent round of transcription. Binding of TFIIA to TBP helps to stabilize TBP binding to the TATA element. TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12). |
Specificity【特异性】 | This rabbit polyclonal antibody detects Transcription initiation factor IIA subunit 1 in human cells. It targets an epitope within 15 amino acids from the C-terminal region. |
Immunogen【免疫原】 | Epitope: C-terminus KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Transcription initiation factor IIA subunit 1. |
Application【应用】 | Anti-Transcription Initiation Factor IIA subunit 1, Cat. No. ABE1797, is a highly specific rabbit polyclonal antibody that targets Transcription initiation factor IIA subunit 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. Research Category Epigenetics & Nuclear Function Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Transcription initiation factor IIA subunit 1 in 10 µg of RAW264.7 cell lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Transcription initiation factor IIA subunit 1 in human brain and human testis tissue. |
Quality【质量】 | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected Transcription initiation factor IIA subunit 1 in 10 µg of HeLa cell lysate. |
Physical form【外形】 | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | mouse (based on 100% sequence homology), rat (based on 100% sequence homology) |
packaging【包装】 | antibody small pack of 25 μg |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NP_056943 |
UniProt accession no.【UniProt登记号】 | P52655 |
shipped in【运输】 | ambient |
Gene Information | human ... GTF2A1(2957) |
产品说明
Target description【目标描述】 | ~55 kDa observed; 41.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |