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Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416)
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | PAb416, monoclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity | SV40-infected cells |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | do not freeze |
isotype【同位素/亚型】 | IgG2a |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 200 μg in Glass bottle Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Recognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells. This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) is validated for use in Affinity Purification, Immunoblotting, IF, IP, Paraffin Sections for the detection of SV40 T Antigen (Ab-2). SV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene. Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen. |
Immunogen【免疫原】 | Epitope: Within amino acids 83-128 purified SV40 large T-antigen |
Application【应用】 | Affinity Purification (use Cat. No. DP02A) Immunoblotting (1-5 µg/ml, see application references) Immunofluorescence (1-5 µg/ml) Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A) Paraffin Sections (see application references) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5. |
Analysis Note【分析说明】 | Positive Control SV80 or SV-T2 cells Negative Control 3T3 cells |
Other Notes【其他说明】 | The agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems. DeCaprio, J.A., et al. 1988. Cell54, 275. Whyte, P., et al. 1988. Nature334, 124. Mitchell, P.J., et al. 1987. Cell50, 847. Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001. Simanis, V. and Lane, D.P. 1985. Virol.144, 88. Mann, R.S. and Carroll, R.B. 1984. Virology138, 379. Sarnow, P., et al. 1982. Cell28, 387. Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41. Lane, D.P. and Crawford, L.V. 1979. Nature278, 261. Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754. Tooze, J. 1973. Cold Spring Harbor, New York. Tegtmeyer, P. 1972. J. Virol.10, 591. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |