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Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416)

产品编号:4087613
规格:liquid, clone PAb416, Calbiochem®
包装规格:200 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4087613200 μG5850
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
PAb416, monoclonal
form【形式】
liquid
contains【包含】
≤0.1% sodium azide as preservative
species reactivity
SV40-infected cells
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
do not freeze
isotype【同位素/亚型】
IgG2a
shipped in【运输】
wet ice
storage temp.【储存温度】
2-8℃
packaging【包装】
200 μg in Glass bottle
Please refer to vial label for lot-specific concentration.
基本信息
General description【一般描述】
Recognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells.
This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) is validated for use in Affinity Purification, Immunoblotting, IF, IP, Paraffin Sections for the detection of SV40 T Antigen (Ab-2).
SV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen.
Immunogen【免疫原】
Epitope: Within amino acids 83-128
purified SV40 large T-antigen
Application【应用】
Affinity Purification (use Cat. No. DP02A)

Immunoblotting (1-5 µg/ml, see application references)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)

Paraffin Sections (see application references)
Warning【警告】
Toxicity: Standard Handling (A)
Physical form【外形】
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analysis Note【分析说明】
Positive Control
SV80 or SV-T2 cells
Negative Control
3T3 cells
Other Notes【其他说明】
The agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
DeCaprio, J.A., et al. 1988. Cell54, 275.
Whyte, P., et al. 1988. Nature334, 124.
Mitchell, P.J., et al. 1987. Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.
Simanis, V. and Lane, D.P. 1985. Virol.144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology138, 379.
Sarnow, P., et al. 1982. Cell28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.
Tooze, J. 1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol.10, 591.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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