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产品别名
Anti-LC3 Antibody
Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 li
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. |
Immunogen【免疫原】 | Linear peptide corresponding to human LC3. |
Application【应用】 | Research Sub Category Apoptosis - Additional Western Blot Analysis: A 1;1,000 dilution from a representative lot detected LC3 in 10 µg of mouse embryonic fibroblast tissue lysate and MCF-7 cell lysate. Research Category Apoptosis & Cancer Anti-LC3 Antibody is an antibody against LC3 for use in Western Blotting. |
Quality【质量】 | Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: A 1;1,000 dilution of this antibody detected LC3 in 10 µg of NIH/3T3 cell lysate. |
Linkage【联系】 | Replaces: MABC177; MABC176 |
Physical form【外形】 | Unpurified Rabbit polyclonal serum containing 0.05% sodium azide. |
Analysis Note【分析说明】 | Control NIH/3T3 cell lysate |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | rat, mouse, human |
technique(s) | western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_115903 |
UniProt accession no.【UniProt登记号】 | Q9H492 |
shipped in【运输】 | wet ice |
Gene Information | human ... MAP1LC3A(84557) |
产品说明
Target description【目标描述】 | ~16 kDa observed. LC3 can occur as a doublet around ~15 kDa, indicating the presence of both LC3-I & LC3-II. An uncharacterized band may appear at ~39 and ~55 kDa in some lysates. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |