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Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | FBN11, monoclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity | human, mouse, rat |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | do not freeze |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections). Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue. Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin. |
Immunogen【免疫原】 | recombinant human fibronectin |
Application【应用】 | Frozen Sections (0.5-1 µg/ml) Immunoblotting (0.5-1 µg/ml) Immunofluorescence (see comments) Immunoprecipitation (2 µg/mg protein lysate) Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Analysis Note【分析说明】 | Positive Control SW 156 cells or normal kidney tissue |
Other Notes【其他说明】 | Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems. Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355. Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9. Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252. Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47. Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517. Pytela, R., et al. 1985. Cell40, 191. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |