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Anti-OFD1 Antibody
产品编号: | 4087545 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-OFD1 Antibody
Oral-facial-digital syndrome 1 protein homolog, OFD1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | The cilium is a cell surface projection found in many vertebrate cells and it is required to transduce signals important for development and tissue homeostasis. OFD1 is important in cilium development, and thus it is important in cellular development and signaling. OFD1 is a component of the centrioles and appears to control the length of the centrioles in mother-daughter cells. OFD1 also plays an important role in the WNT signaling pathway and in the formation of the left-right axis in embryos. OFD1 is expressed in the cytoplasm during development in most tissues but can be found in the nucleus as well, and it is known to interact with proteins important in chromatin remodeling as well. At least one X-linked genetic mutation is associated with defects in the OFD1 gene. It is a form of oroffaciodigital syndrome characterized by abnormalities in the oral cavity, face, digits and other disfigurements that is lethal in males. |
Immunogen【免疫原】 | Epitope: C-terminus Linear peptide corresponding to the C-terminus of mouse OFD1. |
Application【应用】 | This Anti-OFD1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of OFD1. Research Sub Category Apoptosis - Additional Western Blotting Analysis: A representative lot detected OFD1 in untreated and autophagy-induced/serum-starved mouse MEFs (Tang, Z., et al. (2013). Nature. 502:254-257). Western Blotting Analysis: A representative lot detected OFD1 in wild-type female mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117). Western Blotting Analysis: A representative lot detected OFD1 in male E10.5 knock-out mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117). Immunocytochemistry Analysis: A representative lot detected OFD1 in PCM1 knockdown U2OS cells & untreated and serum-starved/autophagy-induced U2OS cells where OFD1 partially colocalizes with LC3 (Tang, Z., et al. (2013). Nature. 502:254-257). Research Category Apoptosis & Cancer |
Quality【质量】 | Evaluated by Western Blotting in U2OS cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OFD1 in 10 µg of U2OS cell lysate. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | mouse, human |
technique(s) | immunocytochemistry: suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_803178 |
UniProt accession no.【UniProt登记号】 | Q80Z25 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~120 kDa observed. Uncharacterized bands may be observed in some lysates at 45 kDa, 33 kDa & 28 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |