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Anti-Dopamine D4 Receptor Rabbit pAb
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | lyophilized |
does not contain【不包含】 | preservative |
species reactivity | rat, human |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze |
isotype【同位素/亚型】 | IgG |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 100 μL in Plastic ampoule |
基本信息
General description【一般描述】 | Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC. Recognizes the ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor. |
Immunogen【免疫原】 | a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor Human |
Application【应用】 | ELISA (see comments) Immunoblotting (1:500) Immunohistochemistry (1:10,000) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | Undiluted serum. |
Reconstitution【重悬】 | Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution. |
Analysis Note【分析说明】 | Positive Control Rat brain |
Other Notes【其他说明】 | Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325. This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer). |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 2 |