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Anti-LRRK2 Antibody
产品编号: | 4087446 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-LRRK2 Antibody
Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin, JH5514
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | LRRK2 (Leucine-rich repeat serine/threonine-protein kinase 2) is a multifunctional protein belonging to the ROCO family of proteins. LRRK2 contains multiple domains including a ROC (Ras of Complex protein) domain that binds and hydrolyzes GTP, and a kinase domain involved in the phosphorylation of myelin basic protein (MBP), mosein, ezrin, and radixin. LRRK2 itself undergoes autophosphorylation and is targeted by other kinases that modify multiple serine residues at its N-terminus; It is constitutively phosphorylated at Ser935 and maintains this state by interaction with the 14-3-3 protein. Although the kinases upstream of LRRK2 have not been definitively identified, previous studies have suggested that this group of kinases may include PKA. It also contains multiple leucine-rich repeat motifs that facilitate interaction with a range of proteins including PARK2 and PRDX3. Mutations in LRRK2, most of which have enhanced its kinase activity, may contribute to the development of late-onset familial Parkinson’s disease; however, the precise molecular mechanism of LRRK2-induced Parkinson’s disease is still unclear. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human LRRK2. |
Application【应用】 | This Anti-LRRK2 Antibody is validated for use in WB, IH(P) for the detection of LRRK2. This antibody is known as JH5514 in scientific publications. Research Category Neuroscience Research Sub Category Neurodegenerative Diseases Western Blot Analysis: A representative lot was used by an independent laboratory in HEK-293T cells transiently transfected with myc-tagged WT or mutant LRRK2. (West, A., et al. (2005). PNAS. 102(46):16842–16847.) Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in neurons from mouse forebrain tissue. (Higashi, S., et al. (2007). Journal of Neurochemistry. 100:368–381.) Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in transfected HEK-293T cells. (West, A., et al. (2005). PNAS. 102(46):16842–16847.) Immunofluorescence Analysis: A representative lot was used by an independent laboratory in transfected HEK-293T cells. (West, A., et al. (2005). PNAS. 102(46):16842–16847.) |
Quality【质量】 | Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected LRRK2 in 10 µg of mouse brain tissue lysate. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control Mouse brain tissue lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human, mouse |
technique(s) | immunofluorescence: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_940980 |
UniProt accession no.【UniProt登记号】 | Q5S007 |
shipped in【运输】 | wet ice |
Gene Information | human ... LRRK2(120892) |
产品说明
Target description【目标描述】 | ~290 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |