Anti-phospho-IRS1 (Ser302 mouse/ Ser307 human) Antibody, clone 8.1.2

32160702

NA.41

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

This antibody recognizes IRS1 phosphorylated at Ser302 (mouse) and Ser307 (human).
Predicted to cross-react with many other species based on 100% sequence homology with immunogen.

Synthetic linear peptide corresponding to amino acids at and around phosphorylated Ser302 of mouse IRS1.
Epitope: Phosphorylated Ser302

Anti-phospho-IRS1 (Ser302 mouse/ Ser307 human) Antibody, clone 8.1.2 is an antibody against phospho-IRS1 (Ser302 mouse/ Ser307 human) for use in IH, IF, IP & WB.
Research Category
Metabolism

Signaling
Immunocytochemistry:
IR/IRS transfected CHO cells were stained with a 1:500 dilution of the antibody. A seconday anti-mouse Cy3 conjugate was used for visualizaion.

Immunoprecipition:
This antibody was used at a 1:1,000 to immunoprecipitate IRS1 from 5L/mL IR/IRS1 transfected CHO cells (performed by an independent lab).

Research Sub Category
Insulin/Energy Signaling

Glucose/Glycogen Metabolism

Metabolic Hormones & Receptors

Evaluated by western blot on IRS1 transfected CHO +/- Insulin lysate.

Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 phosphorylated on Ser302 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.

Format: Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified antibody

primary antibodies

8.1.2, monoclonal

mouse, monkey, bovine, pig, human, canine, rat

immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NP_005535.1

P35568

wet ice

~185 kDa

Stable for 1 year at 2-8ºC from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1