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Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10

产品编号:4085185
规格:clone 3H10, Upstate®, from mouse
包装规格:100 μg
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4085185100 μg7360
产品别名

Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10

H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Specificity【特异性】
Broad species cross-reactivity is expected.
Histone H3 phosphorylated at Ser10.
Application【应用】
Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10 is a mouse monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as H3S10p, is published in peer reviewed journals and has been validated in FC, ICC, IF, PIA, WB, Mplex.
Flow Cytometry:
This antibody has been reported by an independent laboratory to detect phosphorylated histone H3 using flow cytometry.

Beadlyte Histone-Peptide Specificity Assay:
1:1,000 to 1:81,000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.


Immunocytochemistry:
0.2 μg/mL of a previous lot showed positive chromosome immunostaining for mitotic A431 and HeLa cells fixed with 95% ethanol and 5% acetic acid and permeabilized with 0.1% Triton X-100.

Peptide Inhibition Analysis:
Detection of histone H3 in immunoblots of colcemid treated HeLa acid extracts by anti-phospho-Histone H3 (Ser10) was diminished by 10 μM of histone H3 peptide containing phospho-serine 10, but not by peptides containing phospho-serine 28 or an unmodified histone H3 sequence (Figure C).

Immunofluorescence:
Quality【质量】
Routinely evaluated by western blot acid extracted proteins from mitotic HeLa cells treated with colcemid (Catalog # 17-306).

Western Blot Analysis:
0.05-0.5 μg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid (Catalog # 17-306), but not unmodified recombinant Histone H3 (Catalog # 14-494) (Figure A).
Physical form【外形】
Format: Purified
Purified mouse IgG1k in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
Protein G Purified
Analysis Note【分析说明】
Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts.
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
3H10, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NM_003493
UniProt accession no.【UniProt登记号】
Q16695
shipped in【运输】
wet ice
Gene Information
human ... HIST1H3F(8968)
产品说明
Target description【目标描述】
17 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at 2 to 8°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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