Anti-FOXA1 Antibody, clone 2F83

32160702

NA.41

FOXA1 (HNF3A, Hepatocyte nuclear factor 3-alpha) is member of the Forkhead family of winged-helix transcription factors. It is known to be involved in the development and differentiation of several organs including liver, kidney, pancreas, lung, prostate and mammary gland (Lupien, 2008). High expression of FOXA1 is often seen in tumors arising from both the prostate and breast where it is believed to interact with either the androgen receptor or estrogen receptor α, in each of the tumor type respectively (Lupien, 2008). In the case of ER, FOXA1 has gained notice because of its interactions with the cis-regulatory regions in heterochromatin and because it enhances the interaction of ER with chromatin (Badve, 2007).

This antibody detects human FOXA1 (HNF3α).
Other species have not been tested.

Recombinant human GST-FOXA1 protein encompassing amino acids 7-86.
Epitope: N-terminus

Research Sub Category
Transcription Factors
Immunohistochemisty:
Independent labs have verified this clone to work in paraffin-embedded IHC (IH(P)) of various mouse tissues including both embryonic and adult brain, adult digestive tract, adult respiratory tract, and adult urinary tract. Staining was performed using the antibody at a 1:2,000 dilution, but dilutions and conditions must be user optimized.

Research Category
Epigenetics & Nuclear Function
Use Anti-FOXA1 Antibody, clone 2F83 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXA1 also known as Forkhead box protein A1, hepatocyte nuclear factor 3 alpha.

Evaluated by western blot on HeLa nuclear extracts.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect FOXA1 in HeLa nuclear extract.

Format: Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified antibody

2F83, monoclonal

human, mouse

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG1κ

NP_937987

P55317

wet ice

49 kDa

Stable for 1 year at 2-8ºC from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1