Nek6, Active human

MFCD07370972

NA.32

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 ℃ using a final concentration of 50 μM [³²P] ATP.

Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

100

expressed in E. coli

≥80% (SDS-PAGE)

buffered aqueous glycerol solution

≥10 units/mg protein

protein apparent mol wt ~58 kDa

Q9HC98

dry ice

−70℃

human ... NEK6(10783)

12 - Non Combustible Liquids

WGK 1