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Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9
产品别名
Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9
Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK 2, Mitogen-activated protein kinase 2, extracellular signal-regulated kinase-2, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | ERK2 is activated by MEK2, which phosphorylates neighboring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transeitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus. |
Specificity【特异性】 | This antibody recognizes MAP kinase encoded by the mapk gene, Mr 42 kDa. Other species cross reactivity is unknown. |
Immunogen【免疫原】 | HPLC purified murine recombinant MAP Kinase. Clone 1B3B9 |
Application【应用】 | Research Sub Category MAP Kinases Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 is an antibody against MAP Kinase 2/Erk2 for use in IP & WB. Immunoprecipitation: 4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase. Research Category Signaling |
Quality【质量】 | Routinely evaluated in RIPA lysates from human A431 carcinoma cells, mouse 3T3 fibroblasts, or rat L6 cells. Western Blot Analysis: 0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells. |
Linkage【联系】 | Replaces: 04-349 |
Physical form【外形】 | Protein G Chromatography Format: Purified Purified mouse monoclonal IgG2a in buffer containing PBS with 0.05% sodium azide. Store frozen at -20°C, avoid freeze thaw cycles. |
Analysis Note【分析说明】 | Control A431, HeLa and NIH/3T3 cell lysates Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 1B3B9, monoclonal |
species reactivity | avian, rat, mouse, human |
manufacturer/tradename | Upstate® |
technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2a |
NCBI accession no.【NCBI登记号】 | NM_002745 |
UniProt accession no.【UniProt登记号】 | P28482 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | 42 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |