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Anti-Kaiso Antibody, clone 6F
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
Specificity【特异性】 | Kaiso |
Immunogen【免疫原】 | Recombinant N-terminal GST and His-tagged fusion protein corresponding to residues 103-584 of murine Kaiso |
Application【应用】 | Research Category Epigenetics & Nuclear Function Research Sub Category Transcription Factors Detect Kaiso with Anti-Kaiso Antibody, clone 6F (Mouse Monoclonal Antibody), that has been shown to work in EMSA, IP, WB & IHC. |
Quality【质量】 | Immunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells. Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999). Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001). Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001). |
Physical form【外形】 | Protein G Chromatography 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. Format: Purified |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 6F, monoclonal |
species reactivity | human, monkey, mouse, chicken, rat, canine, hamster |
manufacturer/tradename | Upstate® |
technique(s) | electrophoretic mobility shift assay: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1 |
NCBI accession no.【NCBI登记号】 | NM_006777 |
UniProt accession no.【UniProt登记号】 | Q86T24 |
shipped in【运输】 | dry ice |
Gene Information | human ... ZBTB33(10009) |
产品说明
Target description【目标描述】 | 97kDa |
Storage and Stability【储存及稳定性】 | 2 years at -20°C |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |