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Anti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal

产品编号:4084662
规格:clone 62-141-13, Upstate®, from rabbit
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4084662100 μL7360
产品别名

Anti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal

H4, Histone H4, H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
This antibody recognizes histone H4 independant of any post-translational modifications.
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Specificity【特异性】
Broad species cross-reactivity expected based on sequence homology.
Recognizes Histone H4, Mr 10 kDa.
Immunogen【免疫原】
Epitope: a.a. 25-28
KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.
Application【应用】
Epitope Mapping (using Luminex®):
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Anti-Histone H4 Antibody, pan, clone 62-141-13 is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex, ChIP-seq.
Quality【质量】
Routinely evaluated by western blot.

Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)
Physical form【外形】
Format: Purified
Protein A purified
Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.
Analysis Note【分析说明】
Control
Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
Luminex is a registered trademark of Luminex Corp
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
62-141-13, monoclonal
species reactivity
vertebrates, yeast, human, chicken, Saccharomyces cerevisiae
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
multiplexing: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NP_003529
UniProt accession no.【UniProt登记号】
P62805
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
10 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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