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Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal

产品编号:4084661
规格:clone A3S, Upstate®, from rabbit
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4084661100 μL6690
产品别名

Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal

H3, Histone H3, H3 histone, family 3A

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Specificity【特异性】
Broad species cross-reactivity expected due to sequence homology.
Recognizes Histone H3, Mr 17 kDa
Immunogen【免疫原】
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
Application【应用】
Chromatin Immunoprecipitation:
2 μL of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
Use Anti-Histone H3 Antibody, CT, pan, clone A3S (Rabbit Monoclonal Antibody) validated in ChIP, WB to detect Histone H3 also known as Histone H3.
Quality【质量】
Routinely evaluated by western blot analysis.

Western Blot Analysis:
1:2,000-1:20,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Physical form【外形】
Format: Purified
Protein A purified
Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
Analysis Note【分析说明】
Control
Acid extracted proteins from HeLa cells, HeLa nuclear extracts or acid extracted HeLa cells treated with sodium butyrate.
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
A3S, monoclonal
species reactivity
chicken, human, mouse, rat, Saccharomyces cerevisiae, yeast
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NM_003493
UniProt accession no.【UniProt登记号】
Q16695
shipped in【运输】
wet ice
Gene Information
human ... H3F3B(3021)
产品说明
Target description【目标描述】
17 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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