您的位置:首页 > 产品中心 > Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
产品别名
Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
H3, Histone H3, H3 histone, family 3A
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. |
Specificity【特异性】 | Broad species cross-reactivity expected due to sequence homology. Recognizes Histone H3, Mr 17 kDa |
Immunogen【免疫原】 | KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3. |
Application【应用】 | Chromatin Immunoprecipitation: 2 μL of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate. Use Anti-Histone H3 Antibody, CT, pan, clone A3S (Rabbit Monoclonal Antibody) validated in ChIP, WB to detect Histone H3 also known as Histone H3. |
Quality【质量】 | Routinely evaluated by western blot analysis. Western Blot Analysis: 1:2,000-1:20,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells. |
Physical form【外形】 | Format: Purified Protein A purified Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. |
Analysis Note【分析说明】 | Control Acid extracted proteins from HeLa cells, HeLa nuclear extracts or acid extracted HeLa cells treated with sodium butyrate. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | A3S, monoclonal |
species reactivity | chicken, human, mouse, rat, Saccharomyces cerevisiae, yeast |
manufacturer/tradename | Upstate® |
technique(s) | ChIP: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NM_003493 |
UniProt accession no.【UniProt登记号】 | Q16695 |
shipped in【运输】 | wet ice |
Gene Information | human ... H3F3B(3021) |
产品说明
Target description【目标描述】 | 17 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage. |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |