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Anti-Histidine Tagged Antibody, clone HIS.H8
基本信息
eCl@ss | 32160702 |
NACRES | NA.43 |
General description【一般描述】 | His-Tag is a series of at least 6 histidine residues used as a tag motif at the N- or C-terminus of recombinant proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. Its small size also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media. The His Tag epitope is widely used to detect expression of recombinant proteins in bacterial, yeast, insect,and mammalian systems. |
Specificity【特异性】 | Recognizes tag sequence HHHHHH on either the N- or C-terminus of any recombinant fusion protein; no other amino acids surrounding the 6x-His tag sequence are required for the recognition of the 6x-His tag. |
Immunogen【免疫原】 | 6x His synthetic peptide. |
Application【应用】 | Research Sub Category Epitope Tags RNA Binding Protein (RBP) Research Category Epitope Tags & General Use This Anti-Histidine Tagged Antibody, clone HIS.H8 is validated for use in IC, IP, WB for the detection of Histidine Tagged. Immunocytochemistry: 2 μg/mL of a previous lot of antibody showed positive immunostaining for His tagged Akt1/PKBα in transfected HeLa cells. Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate His Tagged proteins. |
Quality【质量】 | Routinely evaluated by western blot and immunocytochemistry on transfected HeLa cells. Western Blot Analysis: 0.5-2 μg/mL of this lot detected His tagged Akt1/PKBα in RIPA lysates of transfected HeLa cells. |
Physical form【外形】 | Purified Format: Purified Purified mouse monoclonal IgG2b in buffer containing PBS, pH 7.4 with 0.05% sodium azide. |
Analysis Note【分析说明】 | Control RIPA lysates of transfected HeLa cells. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
clone【克隆】 | HIS.H8, monoclonal |
species reactivity (predicted by homology) | all |
manufacturer/tradename | Upstate® |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2b |
shipped in【运输】 | dry ice |
产品说明
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |